Cellulite Research Today is a free monthly online journal that collates and summarizes the latest research about Cellulite, including details on treatment, reduction, removal, cures.

Functional binding of human adipose-derived stromal cells: effects of extraction method and hypoxia pretreatment.

Amos PJ, Bailey AM, Shang H, Katz AJ, Lawrence MB, Peirce SM

Department of Biomedical Engineering, University of Virginia, Health System, Charlottesville, VA 22908, USA.

Human adipose-derived stromal cells (hASCs) were evaluated in vitro for their ability to bind vascular adhesion and extracellular matrix proteins to arrest (firmly adhere) under physiological flow conditions. hASCs were flowed through a parallel plate flow chamber containing substrates presenting immobilized type I collagen, fibronectin, E-selectin, L-selectin, P-selectin, vascular cell adhesion molecule-1 (VCAM-1), or intercellular adhesion molecule-1 (ICAM-1) under static and laminar flow conditions (wall shear stress = 1 dyn/cm). hASCs were able to firmly adhere to type I collagen, fibronectin, VCAM-1, and ICAM-1 substrates, but not to any of the selectins. Pretreatment with hypoxia increased the ability of hASCs isolated by liposuction to adhere to VCAM-1 and ICAM-1, but this effect was not seen in cells isolated by tissue excision. These results indicate that hASCs possess the ability to adhere key adhesion proteins, illustrate the importance of hASC harvest procedure, and suggest mechanisms for homing in a setting where interaction with inflamed or injured tissue is necessary.

Published 25 March 2008 in Ann Plast Surg, 60(4): 437-44.
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